Abstract：To explore the influence of the mechanical properties of cell membrane on electroporation, cell membrane was regarded as a hyperelastic material, and an electromechanical coupling model of local single pore on the cell membrane was established in COMSOL. The influence of the elastic strain energy of the cell membrane on the pore formation was analyzed and the difference of pore formation with different elastic modulus of the cell membrane was compared. The results show that the strain energy of the cell membrane increases the energy barrier for transition from hydrophobic pore to hydrophilic pore. The greater the elastic modulus of the cell membrane, the greater the energy barrier is, and the two are linearly related. On the other hand, the increased strain energy stabilizes the pores and prevents them from expanding indefinitely, and the stable size is related to the mechanical properties of the cell membrane and the transmembrane potential. This paper reveals the importance role of the mechanical properties of the cell membrane in electroporation through simulation, which provides a theoretical reference for studying the influence mechanism of cell structure on electroporation.
米彦, 吴晓, 徐进, 郑伟, 马驰. 细胞膜力学性能对电穿孔影响的仿真研究[J]. 电工技术学报, 2022, 37(17): 4326-4334.
Mi Yan, Wu Xiao, Xu Jin, Zheng Wei, Ma Chi. Simulation Study on the Effect of Cell Membrane Mechanical Properties on Electroporation. Transactions of China Electrotechnical Society, 2022, 37(17): 4326-4334.
[1] 吴建光. 利用光镊研究生物细胞的力学行为[D]. 合肥: 中国科学技术大学, 2009. [2] Weaver J C.Electroporation of cells and tissues[J]. IEEE Transactions on Plasma Science, 2000, 28(1): 24-33. [3] 程显, 陈硕, 吕彦鹏, 等. 纳秒脉冲作用下核孔复合体影响细胞核膜电穿孔变化的仿真研究[J]. 电工技术学报, 2021, 36(18): 3821-3828. Cheng Xian, Chen Shuo, Lü Yanpeng, et al.Simulation study on the effect of nuclear pore complexes on cell electroporation under nanosecond pulse[J]. Transactions of China Electrotechnical Society, 2021, 36(18): 3821-3828. [4] Freeman S A, Wang M A, Weaver J C.Theory of electroporation of planar bilayer membranes: predictions of the aqueous area, change in capacitance, and pore-pore separation[J]. Biophysical Journal, 1994, 67(1): 42-56. [5] 唐潇, 姚陈果, 郑爽, 等. 不可逆电穿孔多因素动态电导率模型的研究与分析[J]. 电工技术学报, 2021, 36(14): 2912-2921. Tang Xiao, Yao Chenguo, Zheng Shuang, et al.Establishment and analysis of multi-factor dynamic conductivity model for irreversible electroporation[J]. Transactions of China Electrotechnical Society, 2021, 36(14): 2912-2921. [6] 米彦, 代璐健, 刘权, 等. 靶向金纳米棒联合纳秒脉冲电场对体外A375黑色素瘤2D模拟组织的消融效果研究[J]. 电工技术学报, 2021, 36(18): 3766-3775. Mi Yan, Dai Lujian, Liu Quan, et al.Ablation effects of A375 melanoma cells treated by targeted gold nanorods combined with nanosecond pulsed electric fields[J]. Transactions of China Electrotechnical Society, 2021, 36(18): 3766-3775. [7] 米彦, 刘权, 李盼, 等. 低强度纳秒脉冲电场联合靶向金纳米棒对A375黑色素瘤细胞的杀伤效果研究[J]. 电工技术学报, 2020, 35(12): 2534-2544. Mi Yan, Liu Quan, Li Pan, et al.Study of killing effects of A375 melanoma cells treated by low intensity nanosecond pulsed electric fields combined with targeted gold nanorods[J]. Transactions of China Electrotechnical Society, 2020, 35(12): 2534-2544. [8] 姚陈果, 宁郡怡, 刘红梅, 等. 微/纳秒脉冲电场靶向不同尺寸肿瘤细胞内外膜电穿孔效应研究[J]. 电工技术学报, 2020, 35(1): 115-124. Yao Chenguo, Ning Junyi, Liu Hongmei, et al.Study of electroporation effect of different size tumor cells targeted by micro-nanosecond pulsed electric field[J]. Transactions of China Electrotechnical Society, 2020, 35(1): 115-124. [9] van Uitert I, Le Gac S, van den Berg A. The influence of different membrane components on the electrical stability of bilayer lipid membranes[J]. Biochimica et Biophysica Acta, 2010, 1798(1): 21-31. [10] Thompson G L, Roth C, Tolstykh G, et al.Disruption of the actin cortex contributes to susceptibility of mammalian cells to nanosecond pulsed electric fields[J]. Bioelectromagnetics, 2014, 35(4): 262-272. [11] Muralidharan A, Rems L, Kreutzer M T, et al.Actin networks regulate the cell membrane permeability during electroporation[J]. Biochimica et Biophysica Acta (BBA) - Biomembranes, 2021, 1863(1): 183468. [12] Kim H B, Lee S, Chung J H, et al.Effects of actin cytoskeleton disruption on electroporation in vitro[J]. Applied Biochemistry and Biotechnology, 2020, 191(4): 1545-1561. [13] Sun Sheng, Yin Guangyao, Lee Y K, et al.Effects of deformability and thermal motion of lipid membrane on electroporation: by molecular dynamics simulations[J]. Biochemical and Biophysical Research Communications, 2011, 404(2): 684-688. [14] Hoiles W, Krishnamurthy V, Cranfield C G, et al.An engineered membrane to measure electroporation: effect of tethers and bioelectronic interface[J]. Biophysical Journal, 2014, 107(6): 1339-1351. [15] Hoiles W, Krishnamurthy V, Cornell B.Modelling the bioelectronic interface in engineered tethered membranes: from biosensing to electroporation[J]. IEEE Transactions on Biomedical Circuits and Systems, 2015, 9(3): 321-333. [16] Deng Peigang, Lee Y K, Zhang Tongyi.A nonlinear electromechanical coupling model for electropore expansion in cell electroporation[J]. Journal of Physics D: Applied Physics, 2014, 47(44): 445401. [17] Deng Peigang, Lee Y K, Lin Ran, et al.Nonlinear electro-mechanobiological behavior of cell membrane during electroporation[J]. Applied Physics Letters, 2012, 101(5): 053702. [18] Heyden S, Ortiz M.Investigation of the influence of viscoelasticity on oncotripsy[J]. Computer Methods in Applied Mechanics and Engineering, 2017, 314: 314-322. [19] Neu W K, Neu J C.Mechanism of irreversible electroporation in cells: insight from the models[M]. Berlin, Heidelberg: Springer, 2010. [20] Dastani K, Moghimi Zand M, Hadi Amin, et al.Revealing electrical stresses acting on the surface of protoplast cells under electric field[J]. European Journal of Mechanics - B/Fluids, 2019, 76: 292-302. [21] Karatekin E, Sandre O, Guitouni H, et al.Cascades of transient pores in giant vesicles: line tension and transport[J]. Biophysical Journal, 2003, 84(3): 1734-1749. [22] 董守龙. 高频双极性微秒脉冲电场不可逆电穿孔消融肿瘤的实验与机理研究[D]. 重庆: 重庆大学, 2017. [23] Boyce M C, Arruda E M.Constitutive models of rubber elasticity: a review[J]. Rubber Chemistry and Technology, 2000, 73(3): 504-523. [24] Tan Youhua, Sun Dong, Huang Wenhao.A mechanical model of biological cells in microinjection[C]//2008 IEEE International Conference on Robotics and Biomimetics, Bangkok, Thailand, 2009: 61-66. [25] Skalak R, Tozeren A, Zarda R P, et al.Strain energy function of red blood cell membranes[J]. Biophysical Journal, 1973, 13(3): 245-264. [26] Xu Dong, Kaliviotis E, Munjiza A, et al.Large scale simulation of red blood cell aggregation in shear flows[J]. Journal of Biomechanics, 2013, 46(11): 1810-1817. [27] Kotnik T, Rems L, Tarek M, et al.Membrane electroporation and electropermeabilization: mechanisms and models[J]. Annual Review of Biophysics, 2019, 48: 63-91. [28] Chang D C, Reese T S.Changes in membrane structure induced by electroporation as revealed by rapid-freezing electron microscopy[J]. Biophysical Journal, 1990, 58(1): 1-12. [29] Graybill P M, Davalos R V.Cytoskeletal disruption after electroporation and its significance to pulsed electric field therapies[J]. Cancers, 2020, 12(5): 1132. [30] Latouche E L, Arena C B, Ivey J W, et al.High-frequency irreversible electroporation for intracranial meningioma: a feasibility study in a spontaneous canine tumor model[J]. Technology in Cancer Research & Treatment, 2018, 17: 1533033818785285.
2022, Vol. 37 
