电工技术学报  2020, Vol. 35 Issue (1): 115-124    DOI: 10.19595/j.cnki.1000-6753.tces.181719
电工理论与新技术 |
微/纳秒脉冲电场靶向不同尺寸肿瘤细胞内外膜电穿孔效应研究
姚陈果, 宁郡怡, 刘红梅, 郑爽, 董守龙
输配电装备及系统安全与新技术国家重点实验室(重庆大学) 重庆 400030
Study of Electroporation Effect of Different Size Tumor Cells Targeted by Micro-Nanosecond Pulsed Electric Field
Yao Chenguo, Ning Junyi, Liu Hongmei, Zheng Shuang, Dong Shoulong
State Key Laboratory of Power Transmission Equipment & System Security and New Technology Chongqing University Chongqing 400030 China
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摘要 为深入研究细胞物理特性对微/纳秒脉冲电场诱导的电穿孔效应差异,以三种不同细胞大小及两种不同细胞核大小的肿瘤细胞为研究对象,基于其细胞几何特征建立1:1五层介电及电穿孔数值模型,并观察其在微/纳秒脉冲电场作用下细胞内外膜的荧光消散情况,对微/纳秒脉冲电场诱导细胞内外膜电穿孔效应进行对比分析,研究微/纳秒脉冲电场靶向作用于不同几何尺寸细胞的敏感性差异机理。结果表明,在90个脉冲宽度为100μs、电场强度为1.5kV/cm的微秒脉冲作用下,细胞尺寸越大,细胞外膜穿孔区域、孔密度和荧光消散程度也越大,即诱导的细胞膜电穿孔效果更强。在80个脉冲宽度为400ns、电场强度为15kV/cm的纳秒脉冲作用下,细胞核尺寸越大,其细胞核膜的穿孔区域、孔密度和荧光消散程度也越大,即诱导的细胞核膜电穿孔效果更强。因此,微/纳秒脉冲电场对肿瘤细胞的作用靶区及尺寸大小具有明显的选择性。该研究可为消融不同肿瘤所需的脉冲电场参数提供数据参考与理论支撑。
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姚陈果
宁郡怡
刘红梅
郑爽
董守龙
关键词 脉冲电场电穿孔荧光探针细胞尺寸选择性    
Abstract:In order to further study the differences in electroporation effects induced by micro-nanosecond pulsed electric fields on cell physical properties. In this paper, three different cell sizes and two different nucleus size cell were used as the research object. Based on the cell geometry, a 1:1 five-layer dielectric and electroporation numerical model was established and observed the process fluorescence dissipation of intracellular and extracellular membrane. The electroporation effect on intracellular and extracellular membrane induced by micro-nanosecond pulsed electric field was compared and analyzed and the electrical sensitivity mechanism of micro-nanosecond pulsed electric field targeting different cells was studied. The results show that the larger the cell size, the greater electroporation area of the extracellular membrane and the pore density and the percentage of fluorescence dissipation under the action of microsecond pulses with a pulse width of 100μs and a field strength of 1.5kV/cm. It is show that the electroporation effect of outer membrane is stronger. the larger the size of the nucleus, the larger the electroporation area of the nuclear membrane and the pore density and the percentage of fluorescence dissipation under the action of 80 nanosecond pulses with a pulse width of 400ns and a field strength of 15kV/cm, It is show that the electroporation effect of nuclear membrane is stronger. Therefore, the micro-nanosecond pulsed electric field has obvious selectivity to the target region and size of tumor cells. Through the research in this paper, data reference and theoretical support can be provided for the pulse electric field parameters needed to ablate different tumors.
Key wordsPulsed electric field    electroporation    fluorescent probe    cell size    selectivity   
收稿日期: 2018-11-06      出版日期: 2020-01-17
PACS: TM151  
  TM836  
  Q64  
基金资助:国家自然科学基金青年科学基金项目(51807016)、国家自然科学基金面上项目(51877022)和重庆市博士后项目(XmT2018012)资助
通讯作者: 董守龙 男,1989年生,博士,研究方向为脉冲功率技术及其生物医学应用。E-mail:dsl@cqu.edu.cn   
作者简介: 姚陈果 男,1975年生,教授,博士生导师,研究方向为电气设备在线监测与故障诊断技术、生物医学中的电工新技术及高电压新技术研究。E-mail:yaochenguo@cqu.edu.cn
引用本文:   
姚陈果, 宁郡怡, 刘红梅, 郑爽, 董守龙. 微/纳秒脉冲电场靶向不同尺寸肿瘤细胞内外膜电穿孔效应研究[J]. 电工技术学报, 2020, 35(1): 115-124. Yao Chenguo, Ning Junyi, Liu Hongmei, Zheng Shuang, Dong Shoulong. Study of Electroporation Effect of Different Size Tumor Cells Targeted by Micro-Nanosecond Pulsed Electric Field. Transactions of China Electrotechnical Society, 2020, 35(1): 115-124.
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