微/纳秒脉冲电场靶向不同尺寸肿瘤细胞内外膜电穿孔效应研究

doi:10.19595/j.cnki.1000-6753.tces.181719

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Abstract In order to further study the differences in electroporation effects induced by micro-nanosecond pulsed electric fields on cell physical properties. In this paper, three different cell sizes and two different nucleus size cell were used as the research object. Based on the cell geometry, a 1:1 five-layer dielectric and electroporation numerical model was established and observed the process fluorescence dissipation of intracellular and extracellular membrane. The electroporation effect on intracellular and extracellular membrane induced by micro-nanosecond pulsed electric field was compared and analyzed and the electrical sensitivity mechanism of micro-nanosecond pulsed electric field targeting different cells was studied. The results show that the larger the cell size, the greater electroporation area of the extracellular membrane and the pore density and the percentage of fluorescence dissipation under the action of microsecond pulses with a pulse width of 100μs and a field strength of 1.5kV/cm. It is show that the electroporation effect of outer membrane is stronger. the larger the size of the nucleus, the larger the electroporation area of the nuclear membrane and the pore density and the percentage of fluorescence dissipation under the action of 80 nanosecond pulses with a pulse width of 400ns and a field strength of 15kV/cm, It is show that the electroporation effect of nuclear membrane is stronger. Therefore, the micro-nanosecond pulsed electric field has obvious selectivity to the target region and size of tumor cells. Through the research in this paper, data reference and theoretical support can be provided for the pulse electric field parameters needed to ablate different tumors.

Key words： Pulsed electric field electroporation fluorescent probe cell size selectivity

Received: 06 November 2018 Published: 17 January 2020

PACS:	TM151
	TM836
	Q64

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	Articles by authors
	Yao Chenguo
	Ning Junyi
	Liu Hongmei
	Zheng Shuang
	Dong Shoulong

Cite this article:

Yao Chenguo,Ning Junyi,Liu Hongmei等. Study of Electroporation Effect of Different Size Tumor Cells Targeted by Micro-Nanosecond Pulsed Electric Field[J]. Transactions of China Electrotechnical Society, 2020, 35(1): 115-124.

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https://dgjsxb.ces-transaction.com/EN/10.19595/j.cnki.1000-6753.tces.181719 OR https://dgjsxb.ces-transaction.com/EN/Y2020/V35/I1/115

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