High Repetitive Frequency Pulsed Magnetic Field Generator for Cell Activity Studies
Fan Junxian1,2, Zhang Shaozhe1,2, Li Kexin3, Xiang Wenpei3, Han Xiaotao1,2
1. National High Magnetic Field Center Huazhong University of Science and Technology Wuhan 430074 China; 2. State Key Laboratory of Advanced Electromagnetic Engineering and Technology School of Electrical and Electronic Engineering Huazhong University of Science and Technology Wuhan 430074 China; 3. Institute of Reproductive Health Tongji Medical College Huazhong University of Science and Technology Wuhan 430074 China
Abstract：Cell responses to magnetic fields obey an electromagnetic efficacy window defined by a specific combination of frequency, amplitude and time of exposure. Previous studies suggest that the specific pulsed magnetic field sequences have the potential to modulate the regulation of certain types of cells and tissues. Based on the needs of cell research, it is necessary to develop a programmable pulsed magnetic field generator with a peak magnetic field intensity up to 10 mT, a high stability, a wide range of frequency, a steep pulse edge and a configurable Inter-Stimulus Interval. However, the magnet is a large inductive load, the magnet voltage in the rising edge of the pulse needs to be tens of times higher than that in the flat top stage, which makes it challenge to adjust the magnet current with both high repetitive frequency and high precision. In this paper, a high repetitive frequency pulsed magnetic field generator to solve the above contradiction was developed. Firstly, the structure of the magnet was designed as a double-layer helical coil, and the unevenness of the cell culture area at the center was 2.4%. The ratio of magnetic field to the current in the magnet was 2 mT/A, and the requirements of the power supply were presented based on the magnet parameters. Secondly, a two-stage joint topology consisting of a synchronous Buck circuit and a bridge circuit was proposed. The working principle of the topology was explained and the calculation formulas for the parameters of the main components were derived. Lastly, in order to achieve the smooth transition of the current from the rapid rise to the stable value, the small-signal analysis of the power supply system was performed to obtain the transfer function of the duty cycle and the output current. Using the results, this paper configured a hybrid controller utilizing the time-optimal dynamic response of the hysteresis controller and the strong steady-state tracking capability of the PI controller. The feasibility of the scheme was verified by simulations. Based on these, a portable high repetitive frequency pulsed magnetic field generator with a frequency range of 1 Hz~5 kHz, pulse edge less than 50 μs, adjustable precision of 0.1 mT, and peak intensity up to 10 mT was fabricated. A user-friendly Human-Machine Interface was developed to facilitate the effortless recording of experimental data and monitoring of device status. The ratio of magnetic field to the current was measured in the experiments, and the error was less than 0.15% compared to the theoretical values. This paper tested the magnetic field waveforms for different target values, demonstrating the flexibility and the stability of the device. To mitigate the impact of parasitic capacitance on the magnet at high frequencies, the parasitic capacitance value was calculated followed by the design of a continuous current circuit to suppress the oscillation of the magnet voltage. Finally, biological experiments were carried out to observe the effect of the magnetic field on the fertilization ability and embryo development ability of oocytes from aged mice. Exposed to the 1 mT pulsed magnetic field sequence for 10 minutes, the blastocyst developmental rate increased from 15.15% to 52.94%. These results indicate that the pulsed magnetic field significantly enhances the developmental ability of oocytes in elderly mice.
樊俊显, 张绍哲, 李可欣, 相文佩, 韩小涛. 用于细胞活性研究的高重频脉冲磁场装置[J]. 电工技术学报, 2024, 39(13): 3907-3916.
Fan Junxian, Zhang Shaozhe, Li Kexin, Xiang Wenpei, Han Xiaotao. High Repetitive Frequency Pulsed Magnetic Field Generator for Cell Activity Studies. Transactions of China Electrotechnical Society, 2024, 39(13): 3907-3916.
[1] Berg H.Problems of weak electromagnetic field effects in cell biology 1[J]. Bioelectrochemistry and Bioenergetics, 1999, 48(2): 355-360. [2] Cho H W, Kim S N, Kim K K, et al.Pulsed electromagnetic fields stimulate cellular proliferation in different types of cells[J]. IEEE Transactions on Magnetics, 2016, 52(7): 5000604. [3] Parate D, Franco-Obregón A, Fröhlich J, et al.Enhancement of mesenchymal stem cell chondrogenesis with short-term low intensity pulsed electromagnetic fields[J]. Scientific Reports, 2017, 7: 9421. [4] Parate D, Kadir N D, Celik C, et al.Pulsed electromagnetic fields potentiate the paracrine function of mesenchymal stem cells for cartilage regeneration[J]. Stem Cell Research & Therapy, 2020, 11(1): 46. [5] Crocetti S, Beyer C, Schade G, et al.Low intensity and frequency pulsed electromagnetic fields selectively impair breast cancer cell viability[J]. PLoS One, 2013, 8(9): e72944. [6] Yap J L Y, Tai Y K, Fröhlich J, et al. Ambient and supplemental magnetic fields promote myogenesis via a TRPC1-mitochondrial axis: evidence of a magnetic mitohormetic mechanism[J]. FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology, 2019, 33(11): 12853-12872. [7] Tai Y K, Ng C, Purnamawati K, et al.Magnetic fields modulate metabolism and gut microbiome in correlation with Pgc-1α expression: follow-up to an in vitro magnetic mitohormetic study[J]. FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology, 2020, 34(8): 11143-11167. [8] Wong C J K, Tai Y K, Yap J L Y, et al. Brief exposure to directionally-specific pulsed electromagnetic fields stimulates extracellular vesicle release and is antagonized by streptomycin: a potential regenerative medicine and food industry paradigm[J]. Biomaterials, 2022, 287: 121658. [9] 厉中山, 王春露, 刘洁, 等. 短期低频脉冲磁场诱导经典瞬时感受器电位通道1对肱二头肌最大自主收缩力与力量耐力的影响[J]. 中国组织工程研究, 2023, 27(11): 1796-1804. Li Zhongshan, Wang Chunlu, Liu Jie, et al.Effects of short-term low-frequency pulsed electrical magnetic field-induced classical transient receptor potential channel 1 on maximum voluntary contraction and strength endurance of the biceps brachii[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(11): 1796-1804. [10] 李灿, 宋懿花, 周作建. 一种低频交变磁场发生器的设计与实现[J]. 电子设计工程, 2022, 30(17): 1-6. Li Can, Song Yihua, Zhou Zuojian.Design and implementation of a low frequency alternating magnetic field generator[J]. Electronic Design Engineering, 2022, 30(17): 1-6. [11] 朱汉明, 许伟成, 白文芳. 基于多功能数据采集卡低频电磁场发生器的研制[J]. 中国医学物理学杂志, 2016, 33(11): 1168-1173. Zhu Hanming, Xu Weicheng, Bai Wenfang.Design of low frequency electromagnetic field generator based on multifunction data acquisition card[J]. Chinese Journal of Medical Physics, 2016, 33(11): 1168-1173. [12] Khan M, Sirdeshmukh S P S M A, Reza M Q. Optimal selection of PEMF signal for wound healing system[C]//2017 International Conference on Multimedia, Signal Processing and Communication Technologies (IMPACT), Aligarh, India, 2017: 185-188. [13] Saari M M, Hadi Putera Zaini M A, Ahmad H, et al. An AC magnetometer using automatic frequency switching of a resonant excitation coil for magnetic nanoparticles characterization[C]//2018 9th IEEE Control and System Graduate Research Colloquium (ICSGRC), Shah Alam, Malaysia, 2018: 207-210. [14] 许宁, 米彦, 李政民, 等. 用于电感负载的全固态双极性LTD型脉冲电流发生器[J]. 电工技术学报, 2023, 38(13): 3413-3424. Xu Ning, Mi Yan, Li Zhengmin, et al.All-solid-state bipolar linear transformer drive-type pulse current generator for inductive loads[J]. Transactions of China Electrotechnical Society, 2023, 38(13): 3413-3424. [15] Mohseni M, Rajaei A.Design of alternating magnetic field generator for magnetic fluid hyperthermia research application[J]. Scientia Iranica, 2018, 25(6): 3507-3516. [16] Zeinoun M, Serrano D, Medina P T, et al.Configurable high-frequency alternating magnetic field generator for nanomedical magnetic hyperthermia applications[J]. IEEE Access, 2021, 9: 105805-105816. [17] 陈宇, 蒋兴良, 黄廷帆, 等. 飞机和风机叶片除冰用脉冲线圈结构对瞬态电磁场影响研究[J]. 电工技术学报, 2023, 38(16): 4221-4232. Chen Yu, Jiang Xingliang, Huang Tingfan, et al.Study of the effect of pulsed coil structures on transient electromagnetic fields for aircraft and wind turbine blade de-icing[J]. Transactions of China Electrotechnical Society, 2023, 38(16): 4221-4232. [18] 严来军, 高椿明, 陈霄, 等. 用于瞬态电磁测试的脉冲磁场产生系统的研究[J]. 电子科技大学学报, 2015, 44(3): 456-459. Yan Laijun, Gao Chunming, Chen Xiao, et al.Study of pulsed magnetic field generation system for transient electromagnetic test[J]. Journal of University of Electronic Science and Technology of China, 2015, 44(3): 456-459. [19] 伍健, 何礼高, 付国清. 电流纹波率分析与输出滤波电感的优化设计[J]. 电力电子技术, 2010, 44(5): 67-69. Wu Jian, He Ligao, Fu Guoqing.Analysis of the current ripple ratio and optimal design of the output inductor[J]. Power Electronics, 2010, 44(5): 67-69. [20] 邹铭锐, 曾正, 孙鹏, 等. 基于变电阻驱动的SiC器件开关轨迹协同调控[J]. 电工技术学报, 2023, 38(16): 4286-4300. Zou Mingrui, Zeng Zheng, Sun Peng, et al.Coordinated switching trajectory regulation of SiC device using variable resistance gate driver[J]. Transactions of China Electrotechnical Society, 2023, 38(16): 4286-4300. [21] 黄勇胜, 张建忠, 王宁. 一种SiC MOSFET串扰抑制的谐振辅助驱动电路[J]. 电工技术学报, 2022, 37(12): 3004-3015. Huang Yongsheng, Zhang Jianzhong, Wang Ning.A resonant auxiliary drive circuit for SiC MOSFET to suppress crosstalk[J]. Transactions of China Electrotechnical Society, 2022, 37(12): 3004-3015. [22] Solis C J, Rincón-Mora G A. Stability analysis & design of hysteretic current-mode switched-inductor buck DC-DC converters[C]//2013 IEEE 20th International Conference on Electronics, Circuits, and Systems (ICECS), Abu Dhabi, United Arab Emirates, 2013: 811-814. [23] 李凯迪, 陈特放, 成庶, 等. 基于电流滞环控制的逆变器开路故障诊断及容错控制[J]. 电工技术学报, 2019, 34(增刊2): 596-608. Li Kaidi, Chen Tefang, Cheng Shu, et al.Open circuit fault diagnosis and fault-tolerant control of inverter based on current hysteresis control[J]. Transactions of China Electrotechnical Society, 2019, 34(S2): 596-608. [24] Stefanutti W, Mattavelli P.Fully digital hysteresis modulation with switching-time prediction[J]. IEEE Transactions on Industry Applications, 2006, 42(3): 763-769. [25] 陈轶涵, 韦徵, 龚春英. 平均电流控制型移相全桥DC-DC变换器输出阻抗及控制环路优化设计[J]. 电工技术学报, 2013, 28(4): 43-49. Chen Yihan, Wei Zheng, Gong Chunying.Study of output impedance and control loop optimization for average current mode control phase-shift full bridge DC-DC converter[J]. Transactions of China Electro-technical Society, 2013, 28(4): 43-49. [26] 孟建辉, 吴小龙, 张自力, 等. 三相隔离型AC-DC-DC电源自适应线性自抗扰控制方法及纹波抑制补偿策略[J]. 电工技术学报, 2023, 38(14): 3898-3908. Meng Jianhui, Wu Xiaolong, Zhang Zili, et al.Adaptive linear active disturbance rejection control method and ripple suppression compensation strategy for three-phase isolated AC-DC-DC power supply[J]. Transactions of China Electrotechnical Society, 2023, 38(14): 3898-3908. [27] 袁义生. 电感器分布电容的建模[J]. 华东交通大学学报, 2006, 23(5): 90-93, 101. Yuan Yisheng.Modeling of stray capacitor in inductors[J]. Journal of East China Jiaotong University, 2006, 23(5): 90-93, 101. [28] 张玲, 朱桂金. 丁内酯-Ⅰ对小鼠卵母细胞体外成熟及发育能力的影响[J]. 生殖与避孕, 2005, 25(7): 396-399. Zhang Ling, Zhu Guijin.Effect of butyrolactone Ⅰ on maturation in-vitro and developmental competence of murine immature oocytes[J]. Reproduction and Contraception, 2005, 25(7): 396-399.
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