纳秒脉冲电场联合多壁碳纳米管对皮肤癌细胞活性的剂量效应研究

doi:10.19595/j.cnki.1000-6753.tces.181696

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摘要为了研究多壁碳纳米管（MWCNTs）联合纳秒脉冲电场（nsPEF）影响离体细胞活性的剂量效应,采用CCK-8检测法对比分析皮肤癌A375细胞活性随电场强度E、脉宽τ、脉冲个数N等单因素的变化规律。研究表明,细胞活性随电场强度和脉宽的变化具有阈值效应（S形变化）,而随脉冲个数的变化无明显的阈值效应（指数型变化）。细胞活性随脉冲注入能量E(τN)^0.5的增加呈现S形下降。基于logistic模型利用一元非线性回归分析方法分别拟合得到细胞活性与三个脉冲参数间的函数关系。同时,建立细胞活性与多因素之间的归一化关系。结果表明,MWCNTs的加入不影响细胞活性与脉冲参数间的变化规律,但却明显降低了nsPEF杀伤肿瘤细胞所需要的幅值,从而可以有效提高nsPEF在肿瘤治疗中的电气安全性。

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	米彦
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	杨骐瑜
	唐均英

关键词 ：多壁碳纳米管, 纳秒脉冲电场, 细胞活性, logistic模型, 归一化关系

Abstract：To study the dose effect of the activity of in vitro cells treated by nanosecond pulsed electric field (nsPEF) combined with multi-walled carbon nanotubes (MWCNTs), CCK-8 assay was used to compare and analyze the change law of A375 cell activity with field strength E, pulse width τ and number of pulses N. It is found that cell activity has a threshold effect (S-type change) with field strength and pulse width, and there is no obvious threshold effect (exponential change) with the number of pulses. The cell activity exhibits a S-shape decrease as the pulsed injection energy E(τN)^0.5 increases. By using the logistic model, which is a one-dimensional nonlinear regression analysis method, the functional relationship between cell viability and three pulse parameters is obtained. Moreover, a scaling relationship between cell activity and multiple factors is established. The results show that the addition of MWCNTs does not affect the law between cell viability and pulse parameters, but significantly reduces the nsPEF amplitude required for killing tumor cells, thereby effectively improving the electrical safety of nsPEF in tumor therapy.

Key words： Multi-walled carbon nanotubes nanosecond pulsed electric field cell activity logistic model scaling relationship

收稿日期: 2018-10-24 出版日期: 2019-11-28

PACS:	TM836
	Q64

基金资助:国家自然科学基金（51477022）和重庆市科委自然科学基金（cstc2016jcyjA0500）资助项目

通讯作者: 米彦男,1978年生,教授,博士生导师,研究方向为高电压新技术。E-mail: miyan@cqu.edu.cn

作者简介: 李盼男,1993年生,硕士研究生,研究方向为高电压新技术。E-mail: li-pan@cqu.edu.cn

引用本文:

米彦, 李盼, 刘权, 杨骐瑜, 唐均英. 纳秒脉冲电场联合多壁碳纳米管对皮肤癌细胞活性的剂量效应研究[J]. 电工技术学报, 2019, 34(22): 4849-4857. Mi Yan, Li Pan, Liu Quan, Yang Qiyu, Tang Junying. Dose Effect of the Activity of Skin Cancer Cells Treated by Nanosecond Pulsed Electric Field Combined with Multi-Walled Carbon Nanotubes. Transactions of China Electrotechnical Society, 2019, 34(22): 4849-4857.

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https://dgjsxb.ces-transaction.com/CN/10.19595/j.cnki.1000-6753.tces.181696 https://dgjsxb.ces-transaction.com/CN/Y2019/V34/I22/4849

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