靶向金纳米棒联合纳秒脉冲电场对体外A375黑色素瘤2D模拟组织的消融效果研究

doi:10.19595/j.cnki.1000-6753.tces.200884

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摘要为改善纳秒脉冲电场（nsPEF）治疗肿瘤的电气安全性,在证实靶向金纳米棒联合低强度nsPEF可有效杀伤离体肿瘤细胞的基础上,该文进一步研究该联合治疗方法对体外黑色素瘤2D模拟组织的消融效果。首先,使用叶酸修饰后的靶向金纳米棒结合A375黑色素瘤细胞,并通过暗场显微观察验证了其靶向结合效果。然后,对靶向修饰的A375黑色素瘤2D模拟组织施加不同参数（电压、脉冲宽度和脉冲个数）的nsPEF,通过钙黄绿素试剂进行活细胞染色,在荧光显微镜观察下得到肿瘤细胞的消融图像和消融面积。将Comsol有限元仿真得到的电极附近的电场强度分布结果与消融图像的边界进行拟合,得到不同参数下的阈值电场强度大小。结果表明,在不同脉冲参数下,靶向金纳米棒的加入较单独施加nsPEF时的消融面积增加约11.9%~74.9%,阈值电场强度降低约18.2%~25.0%。该文的研究为靶向金纳米棒联合低强度nsPEF更安全、高效治疗肿瘤的进一步动物实验奠定了基础。

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	米彦
	代璐健
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	唐均英

关键词 ：金纳米棒, 纳秒脉冲电场, 消融面积, 阈值电场强度, 叶酸, A375黑色素瘤细胞

Abstract：It is demonstrated that targeted gold nanorods combined with low-intensity nsPEF can effectively kill tumor cells in vitro. To improve the electrical safety problems of nanosecond pulsed electric field (nsPEF) treatment of tumors, this paper further studied the ablation effect of the combined treatment method on melanoma in vitro 2D simulated tissue. The folic acid modified gold nanorods were used to target A375 melanoma cells, and the target binding effect was observed by dark field microscopy. Then, nsPEF with different parameters (voltage, pulse width and number of pulses) was applied to the targeted modified A375 melanoma 2D simulated tissue, and live cells were stained with calcein reagent to obtain tumor cell ablation image and area under fluorescence microscope observation. The boundary of the ablation image area was fitted with the electric field intensity distribution near the electrodes by COMSOL software, and the field thresholds were obtained under different pulse parameters. Compared with nsPEF alone, nsPEF combined with GNR-PEG-FA increased the ablation area by 11.9%~74.9% and reduced the field threshold by 18.2%~25.0% under different pulse parameters. This paper lays the foundation for further animal experiments on safer and more efficient tumor treatment by the combination of target gold nanorods and low-intensity nsPEF.

Key words： Gold nanorods nanosecond pulsed electric fields ablation area field threshold folic acid A375 melanoma cells

收稿日期: 2020-07-20

PACS:	TM836
	Q64

基金资助:国家自然科学基金（51477022）、中央高校基本科研业务费（NO. 2019CDXYDQ0010）和重庆市科委自然科学基金（cstc2016jcyjA0500）资助项目

通讯作者: 米彦男,1978年生,教授,博士生导师,研究方向为高电压新技术。E-mail: miyan@cqu.edu.cn

作者简介: 代璐健女,1996年生,硕士研究生,研究方向为高电压与绝缘技术。E-mail: 939628005@qq.com

引用本文:

米彦, 代璐健, 刘权, 张倩, 唐均英. 靶向金纳米棒联合纳秒脉冲电场对体外A375黑色素瘤2D模拟组织的消融效果研究[J]. 电工技术学报, 2021, 36(18): 3766-3775. Mi Yan, Dai Lujian, Liu Quan, Zhang Qian, Tang Junying. Ablation Effects of A375 Melanoma Cells Treated by Targeted Gold Nanorods Combined with Nanosecond Pulsed Electric Fields. Transactions of China Electrotechnical Society, 2021, 36(18): 3766-3775.

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https://dgjsxb.ces-transaction.com/CN/10.19595/j.cnki.1000-6753.tces.200884 https://dgjsxb.ces-transaction.com/CN/Y2021/V36/I18/3766

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