纳秒脉冲作用下核孔复合体影响细胞核膜电穿孔变化的仿真研究

doi:10.19595/j.cnki.1000-6753.tces.201332

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Abstract The nanosecond pulsed electric field (nsPEF) can penetrate the cell membrane and target the nuclear membrane to produce electroporation, thereby inducing cell death. However, the existence of a lot of nuclear pore complexes (NPCs) with high conductivity on the nuclear membrane will restrict the development of nuclear electroporation, but it has not been considered in the simulation of nanosecond pulse electroporation. Therefore, in this paper, a five-layer cell dielectric model with NPC in the nucleus was established, and eight NPCs were set at equal distances in different regions of the nuclear membrane. The effect of NPC on nuclear electroporation was studied through the current module and partial differential module of COMSOL. The results showed that: compared with the nuclear membrane electroporation without NPC, the presence of NPC significantly attenuated nuclear membrane electroporation and even changed to a non-electroporation state, and the attenuation degree of nuclear membrane electroporation near NPC was also related to the position of NPC. With the increase in the number of NPC (2~32), the areas with marked attenuation of electroporation on the nuclear membrane also gradually increased. When the number of NPC reached 32, the percentage of areas with attenuation electroporation on the nuclear membrane was 89.93%, the percentages of areas with 50% and 90% attenuation electroporation were 36.00% and 22.94% respectively, and the percentage of areas from electroporation to none-electroporation due to the presence of NPC was 2.54%. In conclusion, NPC can greatly reduce the electroporation on the nuclear membrane, thereby significantly affecting the effect of the nsPEF ablating tumor cells. Therefore, the effect of NPC should be considered when determining the parameters of the nsPEF for the electroporation of nuclear membrane.

Key words： Finite elements electroporation nuclear pore complexes (NPC) dielectric model transmembrane voltage pore density

Received: 30 September 2020

PACS:

TM151

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