纳秒脉冲电场联合多壁碳纳米管对皮肤癌细胞活性的剂量效应研究

doi:10.19595/j.cnki.1000-6753.tces.181696

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Abstract To study the dose effect of the activity of in vitro cells treated by nanosecond pulsed electric field (nsPEF) combined with multi-walled carbon nanotubes (MWCNTs), CCK-8 assay was used to compare and analyze the change law of A375 cell activity with field strength E, pulse width τ and number of pulses N. It is found that cell activity has a threshold effect (S-type change) with field strength and pulse width, and there is no obvious threshold effect (exponential change) with the number of pulses. The cell activity exhibits a S-shape decrease as the pulsed injection energy E(τN)^0.5 increases. By using the logistic model, which is a one-dimensional nonlinear regression analysis method, the functional relationship between cell viability and three pulse parameters is obtained. Moreover, a scaling relationship between cell activity and multiple factors is established. The results show that the addition of MWCNTs does not affect the law between cell viability and pulse parameters, but significantly reduces the nsPEF amplitude required for killing tumor cells, thereby effectively improving the electrical safety of nsPEF in tumor therapy.

Key words： Multi-walled carbon nanotubes nanosecond pulsed electric field cell activity logistic model scaling relationship

Received: 24 October 2018 Published: 28 November 2019

PACS:	TM836
	Q64

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	Mi Yan
	Li Pan
	Liu Quan
	Yang Qiyu
	Tang Junying

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Mi Yan,Li Pan,Liu Quan等. Dose Effect of the Activity of Skin Cancer Cells Treated by Nanosecond Pulsed Electric Field Combined with Multi-Walled Carbon Nanotubes[J]. Transactions of China Electrotechnical Society, 2019, 34(22): 4849-4857.

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https://dgjsxb.ces-transaction.com/EN/10.19595/j.cnki.1000-6753.tces.181696 OR https://dgjsxb.ces-transaction.com/EN/Y2019/V34/I22/4849

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