Ablation Effects of A375 Melanoma Cells Treated by Targeted Gold Nanorods Combined with Nanosecond Pulsed Electric Fields
Mi Yan1, Dai Lujian1, Liu Quan1, Zhang Qian2, Tang Junying2
1. State Key Laboratory of Power Transmission Equipment & System Security and New Technology Chongqing University Chongqing 400030 China; 2. First Affiliated Hospital Chongqing Medical Science University Chongqing 400016 China
Abstract It is demonstrated that targeted gold nanorods combined with low-intensity nsPEF can effectively kill tumor cells in vitro. To improve the electrical safety problems of nanosecond pulsed electric field (nsPEF) treatment of tumors, this paper further studied the ablation effect of the combined treatment method on melanoma in vitro 2D simulated tissue. The folic acid modified gold nanorods were used to target A375 melanoma cells, and the target binding effect was observed by dark field microscopy. Then, nsPEF with different parameters (voltage, pulse width and number of pulses) was applied to the targeted modified A375 melanoma 2D simulated tissue, and live cells were stained with calcein reagent to obtain tumor cell ablation image and area under fluorescence microscope observation. The boundary of the ablation image area was fitted with the electric field intensity distribution near the electrodes by COMSOL software, and the field thresholds were obtained under different pulse parameters. Compared with nsPEF alone, nsPEF combined with GNR-PEG-FA increased the ablation area by 11.9%~74.9% and reduced the field threshold by 18.2%~25.0% under different pulse parameters. This paper lays the foundation for further animal experiments on safer and more efficient tumor treatment by the combination of target gold nanorods and low-intensity nsPEF.
Mi Yan,Dai Lujian,Liu Quan等. Ablation Effects of A375 Melanoma Cells Treated by Targeted Gold Nanorods Combined with Nanosecond Pulsed Electric Fields[J]. Transactions of China Electrotechnical Society, 2021, 36(18): 3766-3775.
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2021, Vol. 36 
