靶向金纳米棒联合纳秒脉冲电场对体外A375黑色素瘤2D模拟组织的消融效果研究

doi:10.19595/j.cnki.1000-6753.tces.200884

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Abstract It is demonstrated that targeted gold nanorods combined with low-intensity nsPEF can effectively kill tumor cells in vitro. To improve the electrical safety problems of nanosecond pulsed electric field (nsPEF) treatment of tumors, this paper further studied the ablation effect of the combined treatment method on melanoma in vitro 2D simulated tissue. The folic acid modified gold nanorods were used to target A375 melanoma cells, and the target binding effect was observed by dark field microscopy. Then, nsPEF with different parameters (voltage, pulse width and number of pulses) was applied to the targeted modified A375 melanoma 2D simulated tissue, and live cells were stained with calcein reagent to obtain tumor cell ablation image and area under fluorescence microscope observation. The boundary of the ablation image area was fitted with the electric field intensity distribution near the electrodes by COMSOL software, and the field thresholds were obtained under different pulse parameters. Compared with nsPEF alone, nsPEF combined with GNR-PEG-FA increased the ablation area by 11.9%~74.9% and reduced the field threshold by 18.2%~25.0% under different pulse parameters. This paper lays the foundation for further animal experiments on safer and more efficient tumor treatment by the combination of target gold nanorods and low-intensity nsPEF.

Key words： Gold nanorods nanosecond pulsed electric fields ablation area field threshold folic acid A375 melanoma cells

Received: 20 July 2020

PACS:	TM836
	Q64

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	Mi Yan
	Dai Lujian
	Liu Quan
	Zhang Qian
	Tang Junying

Cite this article:

Mi Yan,Dai Lujian,Liu Quan等. Ablation Effects of A375 Melanoma Cells Treated by Targeted Gold Nanorods Combined with Nanosecond Pulsed Electric Fields[J]. Transactions of China Electrotechnical Society, 2021, 36(18): 3766-3775.

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https://dgjsxb.ces-transaction.com/EN/10.19595/j.cnki.1000-6753.tces.200884 OR https://dgjsxb.ces-transaction.com/EN/Y2021/V36/I18/3766

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