基于网格传输网络模型的高频纳秒脉冲串作用下单细胞穿孔特性仿真

doi:10.19595/j.cnki.1000-6753.tces.171089

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Abstract To study the perforation characteristics of cells under the action of high frequency nanosecond pulse bursts, a typical two-dimensional single cell system including nuclear membrane was discretized into nodes with the help of Matlab. The mesh transport network model (MTNM) was established, and the bidirectional coupling properties of electrical transport and pore transport were given to the nodes. The voltage of each node and the pore density at different discretization pore radii of each pair of membrane nodes were set to be unknown variables. Hence, the ordinary differential equations were formulated and solved. The dynamic process in single-cell electroporation was simulated when applying 10 high-frequency nanosecond pulse (electric field: 5kV/cm, pulse width: 250ns, rising edge time: 30ns, total simulation time: 10s) to the targeted system. Additionally, the influence of the intra-burst number and the intra-burst frequency of pulses on the perforation features of the inner and outer membranes was also examined. The results show that when multiple pulses are applied, the pore number is basically unchanged, but the pore radius has a cumulative effect. Repeating the pulse train can delay the magnitude of the pore number attenuation to some extent. Increasing the number of pulses in the train can increase the number of outer membrane pores, and does not affect the number of nuclear membrane pores. Increasing the intra-burst frequencies does not affect the number of outer membrane pores, and can greatly increase the number of nuclear membrane pores. The simulation results further enrich the perforation mechanism of pulsed electric field acting on biological cells.

Key words： High-frequency nanosecond pulse bursts mesh transport network model single cell system pore number accumulation effect

Received: 19 July 2017 Published: 26 September 2018

PACS:	TM836
	Q64

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	Mi Yan
	Xu Jin
	Liu Hongliang
	Yao Chenguo
	Li Chengxiang

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Mi Yan,Xu Jin,Liu Hongliang等. Simulation on Perforation Features in a Single-Cell System Exposed to High-Frequency Nanosecond Pulse Bursts Based on Mesh Transport Network Model[J]. Transactions of China Electrotechnical Society, 2018, 33(18): 4217-4230.

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https://dgjsxb.ces-transaction.com/EN/10.19595/j.cnki.1000-6753.tces.171089 OR https://dgjsxb.ces-transaction.com/EN/Y2018/V33/I18/4217

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