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| Pulsed Electrical Field-Assisted Extraction of Tanshinones from Salvia Miltiorrhiza |
| Zhou Cheng, Yan Zeyao, Liu Kefu |
| Department of Light Sources and Illuminating Engineering Fudan University Shanghai 200433 China |
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Abstract The extraction of active ingredients is an essential part in the pharmacy of traditional Chinese medicine. However, traditional extraction methods have the disadvantages of low efficiency and time-consuming. It is necessary to develop new extraction methods. In this paper, a novel Pulsed Electric Field (PEF) method was applied for the extraction of liposoluble tanshinones from salvia miltiorrhiza. Main experimental parameters, including electric field strength, pulse width, pulse number and liquid-solid ratio, were studied as the fat-soluble components, and tanshinone IIA, cryptotanshinone and tanshinone I were selected and analyzed by High Performance Liquid Chromatography (HPLC) to indicate the extraction rate. On this basis, the optimal electric parameter was selected according to the mixed-level orthogonal array design (OAD) method. The results show that under the conditions of 10kV/cm, 1 500 pulses, 5.5μs, L/S ratio 20：1 and 1min processing time, compared with the traditional extraction, PEF method can increase the tanshinone IIA extraction rate by 85% and greatly shorten the extraction time (~min). Under synergistic extraction conditions of PEF and moderate temperature (50℃), it can not only overcome the degradation effect of heat-sensitive tanshinone caused by excessively high temperature, but also improve the solute diffusion rate and extraction efficiency. PEF method has the advantage of high efficiency and environmentally-friendly, which provides a new idea for the extraction of traditional Chinese medicine, especially temperature- sensitive components, and shows good prospects for industrialization.
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Received: 30 June 2020
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2022, Vol. 37 
