低强度纳秒脉冲电场联合靶向金纳米棒对A375黑色素瘤细胞的杀伤效果研究

doi:10.19595/j.cnki.1000-6753.tces.190584

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Abstract To improve the electrical safety associated with nanosecond pulsed electric fields (nsPEFs) in tumor treatment, for the first time, the killing effects of low-intensity nsPEFs combined with folic acid (FA) modified gold nanorods on A375 melanoma cells were investigated. The surface of polyethylene glycol-gold nanorods (GNR-PEG) was modified by folic acid (GNR-PEG-FA) to target A375 melanoma cells, and the binding efficacy was observed by dark field microscopy. After determining the safe concentrations of GNR-PEG and GNR-PEG-FA, the effects of the combined treatment on cell viability and apoptosis were studied by changing electric field strengths (2~8kV/cm) and pulse numbers (15~260). It is found that the combination of low-intensity nsPEFs and GNR-PEG-FA shows the best antitumor efficacy, resulting in lower percentages of viable cells and higher percentages of apoptosis at lower electric field strengths and fewer pulses. This combined treatment enhances the killing effects of A375 melanoma cells and improves the electrical safety of nsPEFs treatment.

Key words： Gold nanorods nanosecond pulsed electric fields cell viability apoptosis folic acid A375 melanoma cells

Received: 14 May 2019

PACS:	TM836
	Q64

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	Mi Yan
	Liu Quan
	Li Pan
	Yang Qiyu
	Tang Junying

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Mi Yan,Liu Quan,Li Pan等. Study of Killing Effects of A375 Melanoma Cells Treated by Low Intensity Nanosecond Pulsed Electric Fields Combined with Targeted Gold Nanorods[J]. Transactions of China Electrotechnical Society, 2020, 35(12): 2534-2544.

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https://dgjsxb.ces-transaction.com/EN/10.19595/j.cnki.1000-6753.tces.190584 OR https://dgjsxb.ces-transaction.com/EN/Y2020/V35/I12/2534

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